Effect of depolarizing agents on choline acetyltransferase and acetylcholinesterase activities in primary cell cultures of spinal cord.

نویسندگان

  • I Ishida
  • T Deguchi
چکیده

In cultured neurons dissociated from the spinal cord of fetal mouse, high concentrations of KCl (47 mM) increased choline acetyltransferase (CAT) activity up to 5.5-fold but suppressed acetylcholinesterase (AChE) activity to less than half the level of control cells. Veratridine (3 microM) also increased CAT activity 1.6-fold and suppressed AChE activity to the same level as that induced by high KCl. The increase of CAT activity by the depolarizing agents was blocked by Ca2+ antagonists (verapamil and high Mg2+) and in a low Ca2+ medium, whereas the suppression of AChE activity by high KCl was restored by the same procedures. The synthesis of radiolabeled acetylcholine from [14C]choline was also enhanced 4-fold by incubating cells in high KCl medium. Although the uptake of L-[3H]leucine and [14C]choline into the cells was slightly enhanced by high KCl medium, neither the total amount of protein nor the incorporation of L-[3H]leucine into protein was increased by high KCl medium. These observations indicate that depolarization increased CAT activity in a specific manner, that the activities of CAT and AChE changed inversely under several conditions, and that the effect of depolarization presumably was mediated by the entry of Ca2+ into neuronal cells. The findings raise the possibility that trans-synaptic input could play a crucial role in the development of the activity of cholinergic neurons in spinal cord.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Deprenyl increases synaptophysin and choline acetyltransferase in rat after sciatic nerve axotomy

Neuroprotective effect of deprenyl on motoneurons of spinal cord after axotomy of peripheral nerves such as sciatic has been well established. Deprenyl is an inhibitor of monoamine oxidase type-B (MAO-B). The main function of this agent is the release of neurotransmitters from pre-synaptic terminals. Acetylcholine is a neurotransmitter that is synthesized by choline acetyltransferase (ChAT) and...

متن کامل

Deprenyl increases synaptophysin and choline acetyltransferase in rat after sciatic nerve axotomy

Neuroprotective effect of deprenyl on motoneurons of spinal cord after axotomy of peripheral nerves such as sciatic has been well established. Deprenyl is an inhibitor of monoamine oxidase type-B (MAO-B). The main function of this agent is the release of neurotransmitters from pre-synaptic terminals. Acetylcholine is a neurotransmitter that is synthesized by choline acetyltransferase (ChAT) and...

متن کامل

Choline acetyltransferase activity of spinal cord cell cultures increased by co-culture with muscle and by muscle-conditioned medium

Activity of the enzyme choline acetyltransferase (CAT), which mediates the synthesis of the neurotransmitter, acetylcholine, was increased up to 20- fold in spinal cord (SC) cells grown in culture with muscle cells for 2 wk. This increase was directly related to the duration of co-culture as well as to the cell density of both the SC and muscle involved and was not affected by the presence of t...

متن کامل

The dynamics of choline acetyltransferase and acetylcholinesterase changes in dog spinal cord during ischemia.

Activities of choline acetyltransferase (CAT) and acetylcholinesterase (AChE) were measured in several regions of the spinal cord, spinal ganglia and the sciatic nerve following ligation of the abdominal aorta just below the renal arteries for 20; 40; 80 and 120 min. Aortic ligation for 40 min produced a significant increase in the activities of both enzymes in the lumbar spinal cord. After lig...

متن کامل

Enhancing effect of Tiliacora triandra leaves extract on spatial learning, memory and learning flexibility as well as hippocampal choline acetyltransferase activity in mice

Objective: The present study investigates the effect of Tiliacora triandra leaf extract on spatial learning, memory, and learning flexibility as well as hippocampal choline acetyltransferase (ChAT) activity in mice.  Materials and Methods: Thirty male ICR mice were randomly divided into three groups including 10% Tween 80, T. triandra 300 mg/kg and T. triandra 600 mg/kg. All administrations wer...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of neuroscience : the official journal of the Society for Neuroscience

دوره 3 9  شماره 

صفحات  -

تاریخ انتشار 1983